Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast

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Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast

Retrograde flow of cortical actin networks and bundles is essential for cell motility and retrograde intracellular movement, and for the formation and maintenance of microvilli, stereocilia, and filopodia. Actin cables, which are F-actin bundles that serve as tracks for anterograde and retrograde cargo movement in budding yeast, undergo retrograde flow that is driven, in part, by actin polymeri...

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Intracellular Motility: Myosin and Tropomyosin in Actin Cable Flow

A new study has found that retrograde flow of budding yeast actin cables is facilitated by myosin II but is inhibited by a specific tropomyosin isoform (Tpm2p). Budding yeast therefore contains a minimal component system for elucidating the mechanistic details of retrograde actin flow.

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Actin filaments assembled at the leading edge of neuronal growth cones are centripetally transported via retrograde F-actin flow, a process fundamental to growth cone guidance and other forms of directed cell motility. Here we investigated the role of myosins in retrograde flow, using two distinct modes of myosin inhibition: microinjection of NEM inactivated myosin S1 fragments, or treatment wi...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2006

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.200609155